TY  - JOUR
T1  - Characterization of intracellular reactive species production stimulated by cold atmospheric pressure plasma irradiation
A1  - Kurita, Hirofumi
A1  - Minamijima, Yoshie
A1  - Takashima, Kazunori
Y1  - 2020///
KW  - Plasma medicine
KW  - atmospheric pressure plasma jet
KW  - intracellular reactive species
KW  - oxidative stress.
JF  - International Journal of Plasma Environmental Science and Technology
VL  - 14
IS  - 3
SP  - e03003
EP  - e03003
DO  - 10.34343/ijpest.2020.14.e03003
UR  - http://ijpest.com/Contents/14/3/e03003.html
N2  - Recent progress in biological applications of cold atmospheric pressure plasmas (CAP) shows that various biological effects are mainly due to intracellular oxidative reactions induced by reactive oxygen and nitrogen species (RONS) produced by CAP. For scientific and practical aspects, intracellular RONS production characteristics stimulated by CAP treatment have to be elucidated. We focused on the difference in intracellular RONS production between CAP irradiation to cell suspension and incubation of cells with CAP-irradiated buffer solution. Flow cytometric assays using RONSreactive probes demonstrated that CAP irradiation to a buffer solution containing A549 cells enhanced the intracellular RONS level. The incubation of the cells with the CAP-irradiated buffer solution also increased the RONS level. Furthermore, the contribution of hydrogen peroxide to the intracellular RONS production was investigated. As a result, injecting a concentrated H2O2 solution with a needle demonstrated that a highly concentrated region of hydrogen peroxide that would be formed by CAP irradiation contributed to enhancing intracellular RONS production.
ER  -