Characterization of intracellular reactive species production stimulated by cold atmospheric pressure plasma irradiation
Hirofumi Kurita*, Yoshie Minamijima, Kazunori Takashima
* The author to whom correspondence should be addressed.
Department of Applied Chemistry and Life Science, Toyohashi University of Technology, Japan
Abstract
Recent progress in biological applications of cold atmospheric pressure plasmas (CAP) shows that various biological effects are mainly due to intracellular oxidative reactions induced by reactive oxygen and nitrogen species (RONS) produced by CAP. For scientific and practical aspects, intracellular RONS production characteristics stimulated by CAP treatment have to be elucidated. We focused on the difference in intracellular RONS production between CAP irradiation to cell suspension and incubation of cells with CAP-irradiated buffer solution. Flow cytometric assays using RONSreactive probes demonstrated that CAP irradiation to a buffer solution containing A549 cells enhanced the intracellular RONS level. The incubation of the cells with the CAP-irradiated buffer solution also increased the RONS level. Furthermore, the contribution of hydrogen peroxide to the intracellular RONS production was investigated. As a result, injecting a concentrated H2O2 solution with a needle demonstrated that a highly concentrated region of hydrogen peroxide that would be formed by CAP irradiation contributed to enhancing intracellular RONS production.
Keywords - Plasma medicine, atmospheric pressure plasma jet, intracellular reactive species, oxidative stress.
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